U.S. Route 301 (US 301) is a spur of U.S. Route 1 running through the South Atlantic States. It currently runs 1,098 miles (1,767.50 km) from Glasgow, Delaware at U.S. Route 40 to Sarasota, Florida. It passes through the states of Delaware, Maryland, Virginia, North Carolina, South Carolina, Georgia, and Florida. It goes through the cities of Annapolis, Maryland; Richmond and Petersburg, Virginia; Rocky Mount, Wilson, and Fayetteville, North Carolina; Florence, South Carolina; Statesboro and Jesup, Georgia; and Ocala, Zephyrhills, Brandon, and Sarasota, Florida.
|South end:||US 41 in Sarasota, FL|
|North end:||US 40 in Glasgow, DE|
|Highway system||NY 104 at the Canadian border in Niagara Falls, NY||States||Florida, Georgia, South Carolina, North Carolina, Virginia, Maryland, Delaware|